Human RNA Binding Motif Protein 8 (RBM8) ELISA Kit

Cat.#:

JL52472

Regulatory Status:

RUO

Alternative:

RBM8A; BOV-1A; BOV-1B; BOV-1C; RBM8B; Y14; ZNRP; ZRNP1; Binder of OVCA1-1; RNA-binding protein Y14; Ribonucleoprotein RBM8A;RNA Binding Motif Protein 8

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

0.156-10ng/mL

Sensitivity:

0.08ng/mL

Specificity:

This kit recognizes Human RBM8 in samples. No significant cross-reactivity or interference between Human RBM8 and analogues was observed.

Sample Type:

Tissue homogenates, cell lysates and other biological fluids

Assay Procedure:

Research Area:

Signal Transduction

Size & Price:

Human A1BG Polyclonal Antibody

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Product Datasheets

Instruction:

Data
Citations
Sequence / 3D

| RBM8

RNA-binding protein 8A is a protein that is encoded by the RBM8A gene.This gene encodes a protein with a conserved RNA-binding motif. The protein is found predominantly in the nucleus, although it is also present in the cytoplasm. It is preferentially associated with mRNAs produced by splicing, including both nuclear mRNAs and newly exported cytoplasmic mRNAs.

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

Concentration (ng/mL)	10	5	2.5	1.25	0.625	0.312	0.156	0
OD	2.03	1.21	0.69	0.41	0.29	0.23	0.19	0.09
Corrected OD	1.94	1.12	0.6	0.32	0.2	0.14	0.1	-

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%

| Recovery

The recovery of Human RBM8 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range(%)	Average Recovery(%)
Tissue Homogenates(n=8)	82-101	96
Cell Lysates(n=8)	92-105	103

| Linearity

Samples were spiked with high concentrations of Human RBM8 and diluted with Universal Diluent to produce samples with values within the range of the assay.

		Tissue Homogenates(n=4)	Cell Lysates(n=4)
1 : 2	Range (%)	84-95	86-96
1 : 2	Average (%)	91	93
1 : 4	Range (%)	89-103	87-108
1 : 4	Average (%)	95	98